Model system to study classical nuclear export signals
نویسندگان
چکیده
منابع مشابه
Nuclear export signals and the fast track to the cytoplasm
One of the defining features of eukaryotic cells is the segregation of RNA biogenesis and DNA replication in the nucleus, separate from the cytoplasmic machinery for protein synthesis. Integration of the activities of the nucleus and cytoplasm requires the continuous transport of proteins, RNAs, and small molecules between these two compartments. Nucleocytoplasmic transport is mediated by nucle...
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Recent investigations have elucidated several molecular pathways for the nuclear import and export of proteins (Kau and Silver, 2003; Weis, 2003) across transport passageways or nuclear pore complexes (NPCs) (Dreger, 2003). The NPC is a large (125 MDa) multimeric protein structure that perforates the nuclear envelope and channels proteins greater than 60 kDa into or out of the nucleus. The cons...
متن کاملHuman topoisomerase IIalpha nuclear export is mediated by two CRM-1-dependent nuclear export signals.
Resistance to chemotherapeutic drugs is a major obstacle in the treatment of leukemia and multiple myeloma. We have previously found that myeloma and leukemic cells in transition from low-density log phase conditions to high-density plateau phase conditions export substantial amounts of endogenous topoisomerase II alpha from the nucleus to the cytoplasm. In order for topoisomerase-targeted chem...
متن کاملNuclear export receptor CRM1 recognizes diverse conformations in nuclear export signals
Nuclear export receptor CRM1 binds highly variable nuclear export signals (NESs) in hundreds of different cargoes. Previously we have shown that CRM1 binds NESs in both polypeptide orientations (Fung et al., 2015). Here, we show crystal structures of CRM1 bound to eight additional NESs which reveal diverse conformations that range from loop-like to all-helix, which occupy different extents of t...
متن کاملNLSdb—major update for database of nuclear localization signals and nuclear export signals
NLSdb is a database collecting nuclear export signals (NES) and nuclear localization signals (NLS) along with experimentally annotated nuclear and non-nuclear proteins. NES and NLS are short sequence motifs related to protein transport out of and into the nucleus. The updated NLSdb now contains 2253 NLS and introduces 398 NES. The potential sets of novel NES and NLS have been generated by a sim...
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ژورنال
عنوان ژورنال: AAPS PharmSci
سال: 2002
ISSN: 1522-1059
DOI: 10.1208/ps040318